Automated HPLC method for determining zinc protoporphyrin ix and protoporphyrin ix in erythrocytes of workers exposed to lead.
نویسندگان
چکیده
منابع مشابه
Zinc protoporphyrin IX concentrations between normal adults and the lead-exposed workers measured by HPLC, spectrofluorometer, and hematofluorometer.
To establish the relationship between Zinc protoporphyrin (ZPP) concentrations and blood lead (PbB) levels and to identify reliable analytical methods of ZPP and Protoporhyrin (PP), blood samples were obtained from 263 office workers without the history of occupational lead exposure and 49 lead-acid battery workers. The mean concentrations of PbB for the normal adults and the battery workers we...
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We describe a method for simultaneously measuring concentrations of coproporphyrin, zinc protoporphyrin IX, and protoporphyrin IX in whole blood by liquid chromatography, with use of reversed-phase ion-pair system, fluorometric detection, and internal standardization. Each analysis requires 10 microL of whole blood and 15 min total analysis time. Analytical recovery ranged from 84 to 92%, day-t...
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In a previous study of secondary lead smelter workers (males), a significant prevalence of low hemoglobin levels (less than 14 g/100 ml) was found; a statistically significant negative correlation between hemoglobin and zinc protoporphyrin was also detected. In the present study serum iron (Fe) levels and total iron binding capacity (TIBC) were included in the investigation of 111 secondary lea...
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The oxidation of protoporphyrinogen IX to protoporphyrin TX in yeast cells is enzyme-dependent. The enzyme, protoporphyrinogen oxidase, associated with purified mitochondria isolated from Saccharomyces cereuisiae was solubilized by sonic treatment in the presence of detergent and partially purified. The molecular weight of the enzyme was 180,000 f 18,000. The purified preparation could be store...
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We report the effects of zinc metal substitution on the molecular dynamics of protoporphyrin-IX in dichloromethane solvent by spectrally resolving the femtosecond photon echo spectrum. We have found that the coherence and population dynamics change due to the presence of Zn metal in the protoporphyrin-IX system. Zinc metal reduces the conformational disorder in the molecular structure of protop...
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ژورنال
عنوان ژورنال: Sangyo Igaku
سال: 1988
ISSN: 0047-1879,1881-1302
DOI: 10.1539/joh1959.30.467